The Bio-desulfurizationbio-desulfurization process is usinguses microorganisms for fossil fuelsfuel sulfur elimination. Bio-desulfurization is an eco-friendly operation that can accompaniment Hydro-desulfurization makingaccompany hydro-desulfurization, thus increasing its efficiency. The rate of bio-desulfurization is still comparatively low likenedwhen compared with the demands of an industrial procedure, and few investigations have focused on procedure evolution. In Bio-desulfurizationbio-desulfurization, microorganisms eliminate sulfur from petroleum without demeaning the heterocyclic structure of the combinationscompounds. Bio-desulfurization runs at the moderate condition, resulting in more lowlower prices, and decreased release, without the creation of unacceptable side-products. In evaluating the application of bio-desulfurization as a bio-refining procedure, pilot scales and bioreactors have been doneimplemented. TheTypically, the findings achieved mayof these studies can be usually used. This study will comprehensively review progress and challenges for a competitive bio-desulfurization towardsto promote its commercialization and engineering aspects causingresulting in ultra-low sulfur petroleum.